Cloning and Expression of the cDNA for Human γ-glutamyl Carboxylase
- 13 December 1991
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 254 (5038) , 1634-1636
- https://doi.org/10.1126/science.1749935
Abstract
The cDNA for human gamma-glutamyl carboxylase, which accomplishes the post-translational modification required for the activity of all of the vitamin K-dependent proteins, was cloned. The enzyme is a 758-residue integral membrane protein and appears to have three transmembrane domains near its amino terminus. The hydrophilic COOH-terminal half of the carboxylase has 19.3 percent identity with soybean seed lipoxygenase. Expression of the cloned cDNA resulted in an increase in carboxylase activity in microsomes of transfected cells compared to mock-transfected cells.Keywords
This publication has 13 references indexed in Scilit:
- Identification and purification to near homogeneity of the vitamin K-dependent carboxylase.Proceedings of the National Academy of Sciences, 1991
- The Structure and Insertion of Integral Proteins in MembranesAnnual Review of Cell Biology, 1990
- The Structure And Insertion Of Integral Proteins In MembranesAnnual Review of Cell and Developmental Biology, 1990
- Role of Vitamin-K-Dependent Proteins in Bone MetabolismAnnual Review of Nutrition, 1988
- Improved tools for biological sequence comparison.Proceedings of the National Academy of Sciences, 1988
- 5-Fluorodeoxyuridine as an alternative to the synthesis of mixed hybridization probes for the detection of specific gene sequences.Proceedings of the National Academy of Sciences, 1988
- Evidence for the glycoprotein nature of vitamin K-dependent carboxylase from rat liverBiochimica et Biophysica Acta (BBA) - General Subjects, 1987
- Vitamin K dependent carboxylase: subcellular location of the carboxylase and enzymes involved in Vitamin K metabolism in rat liverBiochemistry, 1980
- Lipid extraction of tissues with a low-toxicity solventAnalytical Biochemistry, 1978
- A new technique for the assay of infectivity of human adenovirus 5 DNAVirology, 1973