Single strand conformation polymorphism monitoring of 16S rDNA Archaea during start-up of an anaerobic digester

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Abstract
A laboratory-scale continuously mixed anaerobic digester was inoculated with a mix of anaerobic sludge and fed with glucose. The start-up strategy was progressive and chemical analyses were done to evaluate digester performance from day 1 to day 107. In parallel, Archaeal community dynamics were monitored by SSCP analysis of the V3 region of 16S rDNA genes and further characterized by partial sequencing of 16S rDNA genes. At day 1 the inoculum contained at least five distinct Archaeal peaks close to known methanogenic species. The dominant peak was very close to Methanosaeta concilli , the remaining species being members of the Methanobacteriales and Methanomicrobiales . A rapid shift of the Archaeal population was observed during the experiment. At day 21 Methanobacterium formicicum , which was not detected at day 1, became the dominant methanogenic species in the bioreactor and remained so until the end of the experiment.

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