Determination of ivermectin residues in the tissues of Atlantic salmon(Salmo salar)using HPLC with fluorescence detection

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Abstract
A sensitive, accurate and reproducible assay for the determination of residual concentrations of ivermectin in the tissues of farmed Atlantic salmon is described. The assay employs the clean‐up of an acetonitrile extract of salmon tissue on a Bond‐Elut C8 column, followed by derivatization with trifluoroacetic acid anhydride. Following separation using HPLC, 22,23‐dihydroavermectin B1a is determined using fluorescence detection. The limit of detection of the assay is 1 ng per g tissue. Liver, taken from fish treated experimentally with ivermectin, was found to contain higher concentrations of 22,23‐dihydroavermectin B1a than any other tissue examined. The half‐life of 22,23‐dihydroavermectin B1a was similar in all tissues studied, ranging from 89 to 98 degree days.