Chloroplast and Nuclear DNA Content of Cultured Spinach Leaf Discs

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Abstract
The amounts of chloroplast DNA and nuclear DNA in the cells of spinach leaf discs cultutred under different light regimes have been measured. The cellular level of ctDNA increased 10-fold in discs cultured in white light and was accompanied by a 2-fold increase in the cellular level of nuclear DNA. In low intensity green light the cellular level of ctDNA increased 6-fold but was not accompanied by an increase in the level of nuclear DNA. No net DNA synthesis on a per cell basis occurred in discs cultured in darkness. Chloroplasts of uncultured leaf discs contained an average of 83 plastome copies. The number of plastome copies per chloroplast after 6 days culture decreased to 36 copies in darkness, remained almost constant at 73 copies in white light and increased to 215 copies in low intensity green light. These results suggest that ctDNA replication can be independent of cellular levels of nuclear DNA and chloroplast division.

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