EFFECTS OF CARBONYL-COMPOUNDS (4-HYDROXYALKENALS) ORIGINATING FROM THE PEROXIDATION OF LIVER MICROSOMAL LIPIDS ON VARIOUS MICROSOMAL-ENZYME ACTIVITIES OF THE LIVER

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  • 1 January 1980
    • journal article
    • research article
    • Vol. 30  (1) , 99-112
Abstract
Carbonyl compounds released during the NADPH-Fe dependent peroxidation of [rat] liver microsomal lipids and identified as 4-hydroxyalkenals (almost entirely as 4-hydroxynonenal), have no effect on microsomal NADPH-cytochrome c reductase while inhibiting microsomal enzymes (such as glucose 6-phosphatase and aminopyrine demethylase) which are affected by lipid peroxidation. The enzyme activity of NADPH-cytochrome c reductase is unaffected (or even increased) when liver microsomes are allowed to peroxidize in the NADPH-Fe dependent system and, contrary to the other enzymes, is similarly unaffected after CCl4 poisoning, in which peroxidation of membrane lipids of liver endoplasmic reticulum has been unequivocally demonstrated. The effects exerted by lipid peroxidation, or by 4-hydroxyalkenals originating from lipid peroxidation, parallel the effects of CCl4 intoxication in vivo.