ARE ACTIVE AND INACTIVE PHOSPHORYLASES HISTOCHEMICALLY DISTINGUISHABLE?

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Abstract
Some features of the phosphorylase activity derived from the biochemical datas are listed. It is considered worthwhile to assess the results of the histochemical reaction. Possibilities to demonstrate the phosphorylase a, phosphorylase b, and the total phosphorylase activity by the modifications of the original Takeuchi and Kuriaki method are successively discussed. In order to distinguish between the activities of two forms of enzyme it is necessary to block the mutual and spontaneous conversions of the active and inactive phosphorylase which may occur in the course of incubation. The intensity of the reactions performed in those conditions should then be compared with the intensity of the Guha and Wegmann' or Grillo's reactions for the total phosphorylase activity. Applying NaF, and EDTA as the blocking agents, and ATP, MgSO4, AMP as the activators, makes it possible to assess the content of two forms of phosphorylase in the tissue section examined.