Comparing cDNA and oligonucleotide array data: concordance of gene expression across platforms for the NCI-60 cancer cells
Open Access
- 25 November 2003
- journal article
- research article
- Published by Springer Nature in Genome Biology
- Vol. 4 (12) , R82
- https://doi.org/10.1186/gb-2003-4-12-r82
Abstract
Microarray gene-expression profiles are generally validated one gene at a time by real-time RT-PCR. We describe here a different approach based on simultaneous mutual validation of large numbers of genes using two different expression-profiling platforms. The result described here for the NCI-60 cancer cell lines is a consensus set of genes that give similar profiles on spotted cDNA arrays and Affymetrix oligonucleotide chips. Global concordance is parameterized by a 'correlation of correlations' coefficient.Keywords
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