Isolation of Lipoprotein-Acid Mucopolysaccharide Complexes from Fatty Streaks of Human Aorta
- 1 January 1972
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 2 (1) , 83-91
- https://doi.org/10.1080/00327487208061455
Abstract
A method is presented for isolation of 1ipoprotein-acid mucopolysaccharide complexes from fatty streaks of human aorta. The complexes were extracted from fatty streaks with 0.15M NaCl and fractionated by gel filtration followed by ultracentrifugation at densities 1.006 and 1.065- The solvent density 1.065 was achieved by using D2O instead of NaCl which decreased dissociation of the complexes. These studies demonstrated that low and very low density lipoproteins are directly involved in the complexing of lipoproteins with mucopolysaccharides in the matrix of aorta intima.Keywords
This publication has 12 references indexed in Scilit:
- Complexing of serum pre-β- and β-lipoproteins and acid mucopolysaccharidesAtherosclerosis, 1970
- Histological and immunofluorescent studies on the evolution of the human atheromatous plaqueJournal of Atherosclerosis Research, 1968
- Practical Methods for Plasma Lipoprotein Analysis1 1Work supported in part by the U.S. Atomic Energy Commission and by grants (HE06222 and FR00102) from the U.S. Public Health Service.Published by Elsevier ,1968
- Quantitative studies on the complexes formed between aortic mucopolysaccharides and serum lipoproteinsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1967
- Sequestration of Serum Low-Density Lipoproteins in the Arterial Intima by Complex Formation.Experimental Biology and Medicine, 1965
- A modified uronic acid carbazole reactionAnalytical Biochemistry, 1962
- The Influence of Chemical and Physicochemical Nature of Macromolecular Polyanions on Their Interaction with Human Serum β-LipoproteinsPublished by Elsevier ,1960
- INTERACTION OF HUMAN SERUM β-LIPOGLOBULIN WITH POLYANIONSPublished by Elsevier ,1957
- THE DISTRIBUTION AND CHEMICAL COMPOSITION OF ULTRACENTRIFUGALLY SEPARATED LIPOPROTEINS IN HUMAN SERUMJournal of Clinical Investigation, 1955
- Rapid, Accurate Method for Determination of Total Chlolesterol in SerumAnalytical Chemistry, 1953