CHROMOSOME CHANGES IN CELL LINES OF THE BOX TURTLE (TERRAPENE CAROLINA) GROWN AT TWO DIFFERENT TEMPERATURES

Publisher Website
Google Scholar
Abstract
The diploid chromo-some number of this species was 50. The morphology of the chromosomes was described. Karyotype analysis revealed no heteromorphic pair or monosomy, indicating that the females are probably homogametic. Nine cell lines and sublines derived from normal, tissues of 3 T. Carolina were sampled for chromosome analysis. Four lines showed pseudo-diploid modes. Three lines had subdiploid modes (2 with modal chromosome no. 49, 1 with 46). The modes of the remaining 2 lines were at the hypertriploid and hypotetraploid region. The cell lines maintained at 30[degree] C had a higher incidence of polyploidy and a relatively higher degree of variation from the normal karyotype than the cell lines maintained at 23[degree]C. Karyotype analysis in a total of 71 cells from different cell lines indicated that the alterations in the karyotype during in vitro cultivation did not occur at random. Three to 4 chromosome pairs had a relatively higher incidence of change than the other morphologically identifiable chromosomes in the complement.