The role of a cholesta-8,14-dien-3β-ol system in cholesterol biosynthesis
- 1 March 1969
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 111 (5) , 757-761
- https://doi.org/10.1042/bj1110757
Abstract
The biosynthesis of cholesterol from squalene and tritiated water is described. Degradation of the cholesterol indicated that C-15 may be involved in cholesterol biosynthesis. In accordance with this view it is shown that in the conversion of [2RS−3H2]mevalonic acid into cholesterol one of the hydrogen atoms at C-15 is removed. A mechanism for the removal of the 14α-methyl group in steroid biosynthesis that involves the labilization of a C-15 hydrogen atom is outlined. In accordance with the requirement of this scheme it is shown that 4,4′-dimethyl-cholesta-8,14-dien-3β-ol is converted into cholesterol.Keywords
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