Expanding the Genetic Code of Escherichia coli
Top Cited Papers
- 20 April 2001
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 292 (5516) , 498-500
- https://doi.org/10.1126/science.1060077
Abstract
A unique transfer RNA (tRNA)/aminoacyl-tRNA synthetase pair has been generated that expands the number of genetically encoded amino acids in Escherichia coli . When introduced into E. coli , this pair leads to the in vivo incorporation of the synthetic amino acid O -methyl- l -tyrosine into protein in response to an amber nonsense codon. The fidelity of translation is greater than 99%, as determined by analysis of dihydrofolate reductase containing the unnatural amino acid. This approach should provide a general method for increasing the genetic repertoire of living cells to include a variety of amino acids with novel structural, chemical, and physical properties not found in the common 20 amino acids.Keywords
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