Trennung und Reinigung von Pankreaskallikreinen
- 1 January 1962
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 328 (Jahresband) , 15-23
- https://doi.org/10.1515/bchm2.1962.328.1.15
Abstract
Kallikrein from autolysed pig pancreas was purified by fractional methanol precipitation, column electrophoresis and chromatography on DEAE cellulose. It consisted of kallikreins A and B, which have hitherto only been differentiated by a very small difference in electrophoretic mobility. The isoelectric point of a mixture of 81% kallikrein A and 19% kallikrein B lies at pH 4,4; the molecular weight (diffusion and sedimentation) is about 24,000. 1 [mu]g. was equivalent to 0.8 kallikrein units in its effect on blood pressure and hydrolysed 1.9 [mu]Mol. p-toluylsulphonylarginine methyl ester/hour under standard conditions.Keywords
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