Multicenter International Work Flow Study of an Automated Polymerase Chain Reaction Instrument

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Abstract
PCR has the potential of extreme sensitivity, specificity, and diversity. As a consequence, transition of this procedure from research to routine application has been relatively rapid in recent years. This transition has, however, been hampered by the labor-intensive, high-skill requirements of existing protocols. Furthermore, as a number of quality-assessment exercises have demonstrated (1)(2)(3)(4)(5)(6)(7)(8), many of the existing “in-house” developed test procedures are of poor reproducibility in routine application. The introduction of a commercially produced test procedure has improved the reliability of testing, but the procedure has remained a relatively high-skill test method with only certain steps becoming semiautomated.

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