Susceptibility of ribosomes of the tetracycline‐producing strain of Streptomyces aureofaciens to tetracyclines

Abstract
Ribosomes from cells of Streptomyces aureofaciens producing tetracycline antibiotics (Tc‐ribosomes) differ in electrophoretic mobility of ribosomal proteins S2, S10 and L19 from those of the same strain, where the production of tetracyclines was suppressed by changed cultivation conditions (C‐ribosomes). Purified tight vacant couples C‐ and Tc‐ribosomes are equally active in the translation of poly(U). Both types of S. aureofaciens ribosomes are more sensitive to tetracycline and chlortetracycline than ribosomes of Escherichia coli in the Phe‐tRNA binding and the translation of poly(U).