Improved Colorimetric Determination of Cell Wall Chitin in Wood Decay Fungi
- 1 July 1983
- journal article
- research article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 46 (1) , 13-16
- https://doi.org/10.1128/aem.46.1.13-16.1983
Abstract
The Svennerholm modification of the Elson-Morgan method for glucosamine analysis was evaluated for its applicability to the rapid determination of chitin in wood decay fungi. The evaluation included extent of chromogen interference, sensitivity, color stability, and hydrolysis conditions for maximum release of glucosamine from fungal cell walls. With our further modification, the Svennerholm method was shown to be suitable for rapid quantitative determination of fungal chitin without chromatographic separation of hydrolysate chromogens.This publication has 12 references indexed in Scilit:
- Chemical analysis of the hyphal walls of Schizophyllum communePublished by Elsevier ,2003
- An evaluation of the Morgan-Elson assay for 2-amino-2-deoxy sugarsCarbohydrate Research, 1979
- Assay for 2-amino-2-deoxy-D-glucose in the presence of other primary aminesCarbohydrate Research, 1975
- Cell-wall Proteins of Aspergillus niger and Chaetomium globosumJournal of General Microbiology, 1969
- Determination of glucosamine and galactosamine using borate buffers for modification of the Elson-Morgan and Morgan-Elson reactionsAnalytical Biochemistry, 1964
- The determination of 10–100 μmg. quantities of hexosamineBiochemical Journal, 1957
- A MODIFIED COLORIMETRIC METHOD FOR THE ESTIMATION OF N-ACETYLAMINO SUGARSJournal of Biological Chemistry, 1955
- A SPECTROPHOTOMETRIC METHOD FOR THE MICRODETERMINATION OF HEXOSAMINESJournal of Biological Chemistry, 1950
- A colorimetric method for the determination of N-acetylglucosamine and N-acetylchrondrosamineBiochemical Journal, 1934
- A colorimetric method for the determination of glucosamine and chondrosamineBiochemical Journal, 1933