Alteration of mouse cerebellar circuits following methylazoxymethanol treatment during development: Immunohistochemistry of GABAergic elements and electron microscopic study
- 8 July 1987
- journal article
- research article
- Published by Wiley in Journal of Comparative Neurology
- Vol. 261 (2) , 253-265
- https://doi.org/10.1002/cne.902610207
Abstract
Methylazoxymethanol (MAM) injected postnatally affects cerebellar development in mice. A single injection at the fifth postnatal day produces hypogranular cerebella whereas a single injection at birth produces, in addition, a disorderly cytoarchitecture of the folium and alteration of Purkinje cell positioning (Bejar et al.: Exp. Brain Res. 57:279–285, ′85). In the present study we have used immunohistochemistry with anti-GABA immune serum and electron microscopy to further characterize these alterations. In addition to the already-described nonoccupied dendritic spines of Purkinje cells both in mice injected the day of birth and or at the fifth postnatal day, we have observed, in animals injected at birth, the absence of pericellular baskets around Purkinje cells and the presence of heterologous synapses between mossy fibres and Purkinje cell dendrites. These heterologous synapses apparently disappear after postnatal day 20. By using an appropriate timing of MAM injection, different types of hypogranular cerebella, phenocopies of different mutants, can be obtained in large enough number to carry out extensive biochemical studies at each developmental age.Keywords
This publication has 32 references indexed in Scilit:
- New candidates for GABAergic neurons in the rat cerebellum: An immunocytochemical study with anti-GABA antibodyNeuroscience Letters, 1986
- Experimental reorganization of the cerebellar cortex. V. Effects of X-irradiation schedules that allow or prevent cell acquisition after basket cells are formedJournal of Comparative Neurology, 1976
- Experimental reorganization of the cerebellar cortex. V. Effects of early X-irradiation schedules that allow or prevent the acquisition of basket cellsJournal of Comparative Neurology, 1976
- Experimental reorganization of the cerebellar cortex. II. Effects of elimination of most microneurons with prolonged x-irradiation started at four daysJournal of Comparative Neurology, 1973
- Experimental reorganization of the cerebellar cortex. I. Morphological effects of elimination of all microneurons with prolonged x‐irradiation started at birthJournal of Comparative Neurology, 1972
- Postnatal development of the cerebellar cortex in the rat. II. Phases in the maturation of Purkinje cells and of the molecular layerJournal of Comparative Neurology, 1972
- Irradiation of the cerebellum in infant rats with low-level X-ray: Histological and cytological effects during infancy and adulthoodExperimental Neurology, 1971
- Postnatal changes in the concentration and distribution of cholinesterase in the cerebellar cortex of ratsExperimental Neurology, 1970
- Autoradiographic and histological studies of postnatal neurogenesis. III. Dating the time of production and onset of differentiation of cerebellar microneurons in ratsJournal of Comparative Neurology, 1969
- Selective destruction of precursors of microneurons of the cerebellar cortex with fractionated low-dose x-raysExperimental Neurology, 1967