11β-Hydroxysteroid Dehydrogenase Activity of the Human Placenta during Pregnancy
- 1 September 1986
- journal article
- research article
- Published by Georg Thieme Verlag KG in Hormone and Metabolic Research
- Vol. 18 (09) , 638-641
- https://doi.org/10.1055/s-2007-1012393
Abstract
A method for the quantitative estimation of 11β-hydroxysteroid dehydrogenase activity (11β-HSD; EC.1.1.146) in human placental homogenates is described. This method is based on the separation of cortisol and cortisone by high performance liquid chromatography after extraction from homogenates incubated in the presence of cortisol and NADP. 11β-HSD activity (pmol/g wet weight per min) averaged 900 ± 150 (mean ± SEM) at 10 ± 2 weeks of gestation, 915 ± 35 at 17 ± 2 weeks and 790 ± 42 at 40 ± 2 weeks, thus supporting the view that the placenta is an effective barrier to maternofetal cortisol transfer throughout gestation.Keywords
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