Time-dependent changes in the size distribution of distearoylphosphatidylcholine vesicles

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Abstract
The results of transmission electron microscopic and ultracentrifugal studies in the size distributions of sonicated distearoylphosphatidylcholine vesicles are reported. Small vesicles (d .apprxeq. 300 .ANG.) were prepared by sonication of pure 1,2-distearoyl-3-sn-phosphatidylcholine in water and incubated at 4, 21, 40, 53 and 65.degree. C. The vesicle size distributions changed as a function of time at all temperatures below the phase-transition temperature, but remained constant at the transition temperature and above. The sizes of structures to which the small vesicles are converted are the same at all temperatures, although the rates of conversion differ. The primary structures formed are identified as larger vesicles. The rate of loss of small vesicles is found to increase with decreasing temperature. At 4 and 21.degree. C, small vesicles are converted to amorphous material, possibly irregular fragments of neat phase, in addition to being converted to larger vesicles. Trace amounts of an impurity commonly produced in the synthesis of 1,2-distearoyl-3-sn-phosphatidylcholine, 1,3-distearoyl-2-sn-phosphatidylcholine, dramatically reduce the rate of loss of small vesicles at 21.degree. C.

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