CHEMICAL CHARACTERIZATION OF ISOLATED EPIDERMAL DESMOSOMES
Open Access
- 1 November 1974
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 63 (2) , 524-530
- https://doi.org/10.1083/jcb.63.2.524
Abstract
Desmosomes, isolated from cow nose epidermis by a method utilizing citrate buffer pH 2.6 and density gradient centrifugation, have been analyzed and found to contain approximately 76% protein, 17% carbohydrate, and 10% lipid. Nonpolar amino acids predominate in desmosomal protein, representing 456 residues per 1,000. The sialic acid content is 5 nM/mg of protein. The lipid fraction is composed of approximately 40% cholesterol and 60% phospholipids.Keywords
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