Growth of Madin-Darby canine kidney epithelial cell (MDCK) line in hormone-supplemented, serum-free medium.
- 1 July 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (7) , 3338-3342
- https://doi.org/10.1073/pnas.76.7.3338
Abstract
Madin-Darby canine kidney (MDCK) cells can grow in synthetic medium supplemented with 5 factors-insulin, transferrin, prostaglandin [PG] E1, hydrocortisone, and triiodothyronine-as a serum substitute. These 5 factors permit growth for 1 mo. in the absence of serum and a growth rate equivalent to that observed in serum-supplemented medium. Dibutyryl cyclic AMP substitutes for PGE1 in the medium. Potential applications of the serum-free medium are discussed. The medium permits a defined analysis of the mechanisms regulating hemicyst formation by hormones and permits the growth of primary kidney epithelial cell cultures in the absence of fibroblast overgrowth.Keywords
This publication has 9 references indexed in Scilit:
- Density-dependent regulation of growth of BSC-1 cells in cell culture: control of growth by serum factors.Proceedings of the National Academy of Sciences, 1977
- Epidermal growth factor stimulates prostaglandin biosynthesis by canine kidney (MDCK) cellsBiochemical and Biophysical Research Communications, 1977
- Actions of Hormones on the KidneyAnnual Review of Physiology, 1977
- Transepithelial transport in cell culture.Proceedings of the National Academy of Sciences, 1976
- Effects of kidney extract on the folic acid induced proliferation of the rat kidneyExperimentelle Pathologie, 1976
- Role of Cyclic Nucleotides in Growth ControlAnnual Review of Biochemistry, 1975
- STUDIES ON PRIMARY CULTURES OF DIFFERENTIATED FETAL LIVER CELLSThe Journal of cell biology, 1972
- Secretory Activity and Oncogenicity of a Cell Line (MDCK) Derived from Canine KidneyScience, 1969