Quantitation of labeled globin messenger RNA by hybridization with excess complementary DNA covalently bound to cellulose
- 1 May 1976
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 15 (9) , 1844-1847
- https://doi.org/10.1021/bi00654a009
Abstract
A method is described to quantitate labeled [mouse] globin mRNA by hybridization with excess cDNA [complementary DNA] enzymatically polymerized on oligo(dT)-cellulose. In a large excess of cDNA-cellulose the rate of RNA hybridization is dependent on DNA concentration and not on RNA concentration. Nonhybridized RNA can be digested by RNase and washed from the cDNA which is covalently bound to cellulose. This enables the detection of labeled globin mRNA even when present in a proportion as low as 0.02-0.03% of the total RNA.This publication has 2 references indexed in Scilit:
- The synthesis of celluloses containing covalently bound nucleotides, polynucleotides, and nucleic acidsBiochemistry, 1968
- A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membraneJournal of Molecular Biology, 1965