Fiber Optic Fluorescence Scanning in Thin-Layer Chromatography
- 1 August 1994
- journal article
- research article
- Published by Taylor & Francis in Instrumentation Science & Technology
- Vol. 22 (3) , 273-281
- https://doi.org/10.1080/10739149408000455
Abstract
The effectiveness of fluorometric scanning using a fiber optic to analyze substances adsorbed onto solid surfaces is described. The relative efficiencies of signal capture by different widths of monochromator slits were studied. The signal and signal-to-noise ratios were compared using monochromators or long-pass filters. Accuracy and precision of the measurements were compared with and without substraction of the background signal; the detection limits for fluorescein and several phenothiazine compounds were compared under different experimental conditions to explore the range of effectiveness of the system.Keywords
This publication has 7 references indexed in Scilit:
- Image analysis of photochemically derivatized and charge-coupled device-detected phenothiazines separated by thin-layer chromatographyJournal of Chromatography A, 1993
- Thin-layer chromatography and fibre-optic fluorimetric quantitation of thiamine, riboflavin and niacinJournal of Chromatography A, 1993
- Fibre-optic remote sensor for in situ flourimetric quantification in thin-layer chromatographyAnalytica Chimica Acta, 1991
- Development of a Video Densitometer for Thin-Layer ChromatographyInstrumentation Science & Technology, 1990
- Progress in densitometry for quantitation in planar chromatographyJournal of Chromatography B: Biomedical Sciences and Applications, 1989
- Spectral detection in thin-layer chromatography by linear photodiode array spectrometryAnalytical and Bioanalytical Chemistry, 1989
- Some Quantitative Aspects of Scanning Densitometry in High-Performance Liquid ChromatographyJournal of Liquid Chromatography, 1985