Isolation of a Strong Suppressor of Nonsense Mutations in Bacillus subtilis
- 1 May 1976
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 65 (1) , 213-223
- https://doi.org/10.1111/j.1432-1033.1976.tb10408.x
Abstract
By treatment of B. subtilis MO-101-P spoA- met thr- su- with ethyl methanesulfonate, a strong suppressor strain of nonsense mutations, B. subtilis MO-101-P spoA- [met-]+ thr- su+44, was isolated. This strain does not suppress phage .phi.29 mutant susB47, selected on a B. subtilis strain containing the su+3 suppressor isolated by Georgopoulos. A revertant from this mutant, susB610, was isolated, being suppressed by both the su+3 and su+44 suppressor strains. The efficiency of suppression by strain su+44 is about 50%. Apparently strain su+44 contains an amber and strain su+3 an ochre suppressor.This publication has 20 references indexed in Scilit:
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