The outcome of cell mediated lympholysis is influenced by the antibiotics in the culture medium

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Abstract
Antibiotics can interact with polymorphic membrane determinants. As a result, these determinants can no longer react with their corresponding antisera (Claas et al. 1981, 1982). Allogeneic cytotoxic T cells (CTL), which were cultured in the presence of antibiotics, induced significantly stronger lysis against the specific target than CTL which were cultured in the absence of antibiotics. In some cases the CML [cell mediated lympholysis] assay was only positive according to the European Workshop criteria when penicillin was added to the cultures, whereas the assay was negative in the absence of penicillin. When CTL were induced in an MLC [mixed leukocyte culture] culture without penicillin, lysis was higher against the target cell in the absence of penicillin than in the presence of penicillin. By adding penicillin to the effector phase, inhibition of lysis was observed. No difference in 3H-thymidine incorporation was observed between MLC cultures with and without antibiotics. Antibiotics in the culture medium can influence the outcome of CML experiments.

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