A survey of the location, isolation and identification of indoles, pteridines and some unknown active substances in sheep pineals. The possible significance of pteridines for the neuroendocrine control of neoplastic growth

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Abstract
Several indoles with mass spectra identical to those of synthetic melatonin, 5-hydroxyindole-3-acetic acid (5-HIAA), 5-methoxyindole-3-acetic acid (5-MIAA), 5-hydroxytryptophol (5-HTL) and 5-methoxy-tryptophol (5-MTL) could be located, isolated and identified in sheep pineal extracts using rather simple and mild extraction and separation methods. An antigonadotropin, which differs from melatonin, was isolated and partially purified from sheep pineals, using compensatory ovarian hypertrophy after unilateral ovariectomy as a bioassay. Antigonadotropic and gonadotropic compounds which differ from the indoles above mentioned could be located with the same methods.In vitro, these fractions act either on the hypothalamus or on the anterior pituitary. From the paper chromatograhy fraction, acting on the anterior pituitaryin vitro, a compound could be isolated which is most probably identical with biopterin. In our opinion, however, this compound is not responsible for the antigonadotropic activity of the fraction, observedin vitro. Some pineal fractions showing an effect on the activity of the hypothalmus or anterior pituitary of male rats and micein vitro, be also active in some experimental tumour models. A possible relationship between disorders in pteridine metabolism and neoplastic growth is discussed.
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