SUGAR CONTENT OF BLOOD, LYMPH AND ARTIFICIAL PERITONEAL FLUID FOLLOWING INJECTION OF INSULIN*

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Abstract
In 18 expts. on unanesthetized dogs Tyrode''s soln. was instilled into the peritoneal cavity. The sugar content of the blood and of the artificial peritoneal fluid (a.p.f.) was detd. before and at hourly intervals after inj. of insulin. Dosages ranged from 1-5 U. per Kg. Similar expts. were performed on 6 rats. Blood sugar levels fell to 30-40 mg. % within the first 2 hrs. and then levelled off. Glucose content of the a.p.f., isoglycemic at the beginning of the expt., decreased much more slowly and reached the low level of the blood sugar 4 1/2 to 5 1/2 hrs. after insulin inj. Convulsions and coma occurred at this time. In one dog and in the rats convulsions occurred 2 hrs. after inj. of insulin and in these animals equilibrium was established at this early time. In 7 expts. on dogs anesthetized with Nembutal a lymphatic vessel of the hind limb was cannulated and the glucose content of blood and lymph, and in 3 expts. also that of a.p.f., was compared after insulin inj. Blood sugar decreased more rapidly in the anesthetized than in the unanesthetized dogs. Lymph sugar drop lagged behind the blood sugar decrease during the first hr. Equilibrium was established at 1 1/2 hrs. in 3 and at a later point in 4 instances.