HISTOCHEMICAL DEMONSTRATION OF CATECHOLAMINES BY FLUORESCENCE INDUCED BY FORMALDEHYDE VAPOUR

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Abstract
A method is described which makes it possible to demonstrate histochemically the localization of catecholamines, e.g. noradrenaline, and enzyme activity in the same tissue section. Frozen dried tissues are exposed to dry formaldehyde vapour at 50[degree]C for 1 hour, which makes catecholamines visible but does not inactivate most enzymes. Enzymes such as acetylcholinesterase, non-specific cholines-terase, non-specific esterases, lactic dehydrogenases or tetrazolium reductases can thereafter be demonstrated and their distribution compared with that of catecholamines. The method is illustrated by a pair of photomicrographs showing the distribution of noradrenaline and esterase activity in the superior cervical ganglion of the rat.

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