Abstract
In Zea mays L. (cv. XL 72 A) leaves sulphur deficiency caused reduction of soluble protein and chlorophyll contents, whereas ATP sulphurylase (EC 2.7.7.4) and O-acetylserine sulphydrylase (EC 4.2.95.9) activities increased with the increasing of S-deprivation time. The two enzymes exhibited the maximum activity after 5 d (ATP sulphurylase) and 3 d (O-acetylserine sulphydrylase) from the beginning of deprivation period. The activities were differently distributed between mesophyll protoplasts and bundle sheath strands. The results suggest that the activity of the two enzymes may be induced sequentially and differently regulated in the two types of cells.

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