Differential inhibition of various mammalian DNA polymerase activities by ammonium 21‐tungsto‐9‐antimoniate (HPA23)
Open Access
- 1 September 1988
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 176 (2) , 305-310
- https://doi.org/10.1111/j.1432-1033.1988.tb14282.x
Abstract
The 21-tungsto-9-antimoniate ammonium salt (HPA23), known as an antiviral agent, has been shown to be a potent inhibitor of both human and murine DNA polymerase α and murine DNA polymerase γ. HPA23 inhibited the activity of DNA polymerase α in noncompetitive fashion with respect to either deoxynucleotide substrate or nucleic acid template · primer. The Ki of murine DNA polymerase α for HPA23 was determined to be 24 nM. The activity of mouse DNA polymerase γ also was strongly inhibited by HPA23 (Ki, 20 nM), and the mode of inhibition was competitive with respect to the template · primer, (rA)n· (dT)12-18, and noncompetitive to substrate, dTTP. DNA polymerase β and terminal deoxynucleotidyltransferase, however, were relatively resistant to inhibition by HPA23. The observed inhibitions by HPA23 seem to be closely related to the polyanionic property of this drug.This publication has 24 references indexed in Scilit:
- Inhibition of reverse transcriptase activity by 2′,3′-dideoxythymidine 5′-triphosphate and its derivatives modified on the 3′ positionBiochemical and Biophysical Research Communications, 1986
- ANTIMONIOTUNGSTATE (HPA 23) TREATMENT OF THREE PATIENTS WITH AIDS AND ONE WITH PRODROMEThe Lancet, 1985
- Characterization of the RNA dependent DNA polymerase of a new human T lymphotropic retrovirus (lymphadenopathy associated virus)Biochemical and Biophysical Research Communications, 1984
- Correlation between structure of polyoxotungstates and their inhibitory activity on polymerasesBiochemical and Biophysical Research Communications, 1983
- Isolation of a T-Lymphotropic Retrovirus from a Patient at Risk for Acquired Immune Deficiency Syndrome (AIDS)Science, 1983
- ANTIVIRAL COMPOUND EFFECTIVE AGAINST EXPERIMENTAL SCRAPIEThe Lancet, 1979
- Inhibition of Rabies Virus in vitro by the Ammonium-5-Tungsto-2-AntimoniateJournal of General Virology, 1978
- The structure of the heteropolytungstate (NH4)17NA|NaW21Sb9O86|.cntdot.14H2O. An inorganic cryptateJournal of the American Chemical Society, 1976
- Inhibition of RNA-dependent DNA polymerase of murine oncornaviruses by ammonium-5-tungsto-2-antimoniateBiochemical and Biophysical Research Communications, 1975
- Interconversion of Molecular Size of the DNA Polymerase from Rauscher Leukemia VirusIntervirology, 1974