Effect of EDTA and Zinc-Methionine Complex on Zinc Absorption by Rat Intestine

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Abstract
The effects of zinc chelators on 65Zn uptake, absorption and tissue distribution were determined in rats using ligated duodenal loops. 65Zn was supplied as Zn-methionine complex, ZnCl2, ZnCl2 + L-methionine or ZnCl2 + EDTA. The effect of EDTA was also determined in the presence of phytic acid. Absorption of 65Zn was markedly reduced in rats given Zn-methionine complex or ZnCl2 + EDTA. The 65Zn level in tissues (liver, bone, muscle, skin, kidney, and thymus) of rats given ZnCl2 + EDTA was also significantly reduced compared to that of rats given ZnCl2. Reduced absorption due to phytic acid was not improved by EDTA, although EDTA increased mucosal 65Zn retention. High performance gel filtration chromatography showed six 65Zn-binding peaks in the mucosal cytosol of rats given ZnCl2. A seventh peak attributable to Zn-EDTA was observed in cytosol from rats given ZnCl2 + EDTA. A comparable peak in plasma was not observed. Both EDTA and Zn-methionine complex reduced 65Zn-binding to a low-molecular-weight component of mucosal cytosol that was not metallothionein. The results suggest that Zn-EDTA is transported intact from the lumen into mucosal cells but not across the basolateral membrane. The adverse effects of EDTA and Zn-methionine complex on zinc absorption were associated with reduced 65Zn-binding to a component of mucosal cytosol that may be involved in zinc absorption.