Alkaline Phosphatase and Ultrastructural Alterations in Human Osteosarcoma Cells in Tissue Culture

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Abstract
A cloned line of cells from a biologically proven osteosarcoma were found to have lost their initial high levels of alkaline phosphatase on prolonged passage through tissue culture. This biochemical marker of osteogenesis was restored by the addition of 0.5 µg/ml of hydrocortisone-21-phosphate to the tissue culture medium. A rise in the alkaline phosphatase activity was accompanied by an increase in the cellular and nuclear size and an increase in the number of polyribosomes, microfibrils, and microvilli of the cells. The Golgi zone in these cells became more prominent and was associated with an increased number of vesicles. The present investigation indicates that alkaline phosphatase activity is associated with ultrastructural differentiation (modulation) of osteosarcoma cells in tissue culture.

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