The use of Seralute® and Tetralute® for measurement of plasma triiodothyronine and thyroxine in salmonid fishes

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Abstract
There was found to be a marked effect of sample volume on the measured and calculated (i.e. corrected for volume size) levels of thyroxine (T4) and triiodothyronine (T3) in trout plasma when using the Ames Tetralute® and Seralute® assay kits. Freezing and thawing caused a significant (p < 0.001) increase in plasma T4 levels and a significant decrease (p < 0.01) in plasma T3 levels. Further freezing and thawing and plasma storage at – 30 °C for 1 month had no significant effect on measured hormone levels.

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