Rate of Cell Division of Malignant Mouse Melanoma B162

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Abstract
The mitotic rate of transplantable malignant B16 melanoma was investigated in C57BL/6J mice at 10 and 14 days after transplantation, with the use of the colchicine technique. No significant difference in mitotic rate was observed. The B16 melanoma apparently grew at a constant rate by a daily addition of 34 to 36 percent of new cells to its population, which implied a doubling time of 2.8 days. Further, the mitotic rate of metastases in spleen and liver was similar to that of the transplanted primary tumors. Moreover, diurnal fluctuations of mitotic activity did not occur in the B16 melanoma. In many respects, the cell population of this tumor behaved like other neoplastic cell populations whose mitotic rates have been ascertained. The histology of the B16 melanoma is described.