Intramolecular Effects of 125 I Decay in o-Iodotyrosine

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Abstract
As a model for iodinated proteins, 3-[125I]iodo[U-14C]tyrosine was synthesized by the chloramine-T method from [U-14C]tyrosine. The products remaining after the iodine had decayed were characterized chromatographically. A reference system was used to correct for hydrolysis and secondary radiolytic effects. All products due to 125I decay were small polar molecules. After the Auger cascade accompanying 125I decay, a distribution of charge throughout the molecule apparently occurs before disruption of the molecule. A coulombic explosion mechanism with some contributions due to internal radiolysis and charge neutralization is proposed for the destruction of the aromatic ring. Implications for proteins labeled with 125I are discussed.

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