Commercial Levels of Chymosin Production by Aspergillus
- 1 October 1991
- journal article
- research article
- Published by Springer Nature in Bio/Technology
- Vol. 9 (10) , 976-981
- https://doi.org/10.1038/nbt1091-976
Abstract
We have increased the production of bovine chymosin in Aspergillus niger var. awamori to more than one gram per liter of secreted authentic enzyme by combining a mutagenesis protocol with a novel robotic screening program. Analysis of the superior chymosin producing strains indicated that they have enhanced capabilities to secrete extracellular proteins.Keywords
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