Partition Chromatography of Organic Acids in Body Fluids with Silica Gel

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Abstract
1. A method for the extraction of non-α-amino organic acids from urine based on the work of Isherwood is described. 2. The relative position of elution of 42 biologically significant acids from silica gel columns is demonstrated. A number of important acids known to be present in urine are not eluted. 3. The partition chromatography of urinary organic acids reveals the presence of 9 peaks, of which 4 are known to be acetic, formic, lactic, and citric acids. A fifth peak contains glycolic and oxalic acids. 4. The aromatic acids are eluted principally in the first peak, which contains hippuric acids as a major constituent. 5. A minimum of two unknown organic acids are constantly present in relatively large concentrations in urine. Two additional acids appear frequently.

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