Nucleotide and Predicted Peptide Sequence of Feline Interleukin-12 (IL-12)
- 1 January 1997
- journal article
- research article
- Published by Taylor & Francis in DNA Sequence
- Vol. 8 (1-2) , 77-82
- https://doi.org/10.3109/10425179709020889
Abstract
Feline Interleukin-12 (IL-12) is a heterodimeric glycoprotein consisting of two disulfide linked subunits of about 40 kD (p40) and 35 kD (p35). It is a pleiotropic cytokine mediating biological activities on T- and NK-cells. One important function is the induction of a Th1 immune response. Here we report the cloning and sequencing of feline IL-12, the expression of the p40-protein in E. coli and production of monoclonal antibodies. At the nucleotide level, feline IL-12 shows between 87-90%, on the amino acid level between 82-87% identity to the bovine and human IL-12, respectively.Keywords
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