The rat β3‐adrenergic receptor gene contains an intron

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Abstract
We report that the rat β3‐adrenergic receptor (β3‐AR) gene has an intron. The intron starts with an in‐frame stop codon with the result that unspliced transcripts will encode a C‐terminal truncated protein. The reported protein sequences of mouse and human β3‐AR were both deduced from genomic DNA sequences. Given the heterogeneity at the C‐termini of the otherwise highly similar rat, mouse and human sequences, we discuss the intriguing possibility that the β3‐AR gene of the latter two species also contain an intron near the extremity of the open reading frame. A β‐adrenergic receptor (β‐AR) cDNA we have cloned from rat colonic tissue which has a sequence essentially identical to that previously reported for the rat adipose β3‐AR cDNA [(1991) J. Chem. 266, 24053], encodes the spliced version of the β3‐AR.