Transcriptional Mapping: Functional Organization of the Ribosomal and Transfer Ribonucleic Acid Cistrons in the Bacillus subtilis Genome
- 1 August 1969
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 99 (2) , 535-543
- https://doi.org/10.1128/jb.99.2.535-543.1969
Abstract
The transcriptional mapping method developed previously has been used to investigate the functional organization of the ribosomal ribonucleic acid (rRNA) and transfer ribonucleic acid (tRNA) cistrons in the Bacillus subtilis genome. The sensitivities of the syntheses of these RNA species to actinomycin D indicated that (i) large polycistronic groupings of the tRNA cistrons exist, but (ii) the 16 S and 23 S rRNA cistrons are probably transcribed as individual units, and (iii) each 5 S rRNA cistron is part of an operon containing a 23 S rRNA cistron.Keywords
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