INITIAL CHARACTERIZATION OF DRUG-METABOLIZING SYSTEMS IN THE LIVER OF THE NORTHERN PIKE, ESOX-LUCIUS

  • 1 January 1980
    • journal article
    • research article
    • Vol. 8  (2) , 98-103
Abstract
NADPH-cytochrome c reductase [NCCR], cytochrome P-450, benzo[a]pyrene mono-oxygenase [BPMO], epoxide hydratase [EH], and glutathione S-transferase [GST] activities in the liver of the Northern pike (Esox lucius) were measured and partially characterized. The level of these systems in pike liver is between 13.2 and 133% of the corresponding levels in rat liver, with the exception of glutathione S-transferase, whose specific activity in the high-speed supernatant fraction of pike liver is 305% of that is rat liver. Pike liver contains about 23% of the mammalian level of reduced glutathione. Drug metabolizing systems in pike liver are distributed in essentially the same manner in subfractions as the corresponding systems in the liver of mammals. BPMO and EH activities display the expected pH maxima of 7.5 and 9.5, respectively, and have temperature maxima of 37.degree. and 47.degree. C, respectively. NCCR and GST activities are relatively independent of temperature. I.p. treatment of Northern pike with methylcholanthrene induces the benzo[a]pyrene mono-oxygenase activity of liver microsomes 33-fold.

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