Estimation of the sizes of polymorphic C-bands in man by measurement of DNA content of whole chromosomes
- 1 January 1977
- journal article
- research article
- Published by S. Karger AG in Cytogenetic and Genome Research
- Vol. 19 (5) , 250-255
- https://doi.org/10.1159/000130817
Abstract
A method is proposed for estimating the sizes of polymorphic C-bands of human chromosomes by microdensitometric measurement of the DNA content of whole chromosomes. The method requires measurements of: a chromosome known not to be polymorphic, as a standard against which to normalize all measurements; the polymorphic chromosomes of interest; and a homologous polymorphic chromosome lacking the C-band. In an example studied here, the C-band of chromosome 9 was estimated to contain about 0.0296 pg of DNA.Keywords
This publication has 3 references indexed in Scilit:
- Photometric determination of the DNA distribution in the 24 human chromosomes*1Experimental Cell Research, 1977
- Influence of Q- and G-banding on the Feflgen-stainability of human metaphase chromosomesJournal of Molecular Histology, 1977
- The segregation of human chromosome polymorphismsAnnals of Human Genetics, 1976