HPLC Method for the Determination of Chloroquine and its Main Metabolite in Biological Samples
- 1 March 1990
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 13 (6) , 1203-1213
- https://doi.org/10.1080/01483919008049243
Abstract
An ion-pair reversed phase HPLC-method for the determination of chloroquine and desethylchloroquine in plasma,blood and filter-paper-absorbed blood spots is described. Using 75 .mu.l samples, concentrations ranging from 2 to 1000 ng/ml can be measured. Intraday precision as well as interday precision are lower than 6% (RSD). The filter-paper method can be used for the determination of chloroquine and desethylchloroquine in samples originating from areas with a lack of laboratory equipment. The procedure is sensitive enough to be used in monitoring of drug levels as well as for pharmacokinetic studies.Keywords
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