Identification of Some Metabolic Products of 5′‐Deoxy‐5′‐S‐Isobutylthioadenosine, an Inhibitor of Virus‐Induced Cell Transformation

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Abstract
5′‐Deoxy‐5′‐S‐isobutylthiodenosine (iBuS)5′ Ado has been shown to be rapidly degraded to 5‐deoxy‐5‐S‐isobutylthioribose and adenine in procaryotes. In chick embryo fibroblasts there are two metabolic pathways for (iBuS)5′ Ado degradation: (a) oxidative deamination into 5′‐deoxy‐5′‐S‐isobutylthioinosine (the main product) and (b) hydrolysis into 5‐deoxy‐5‐S‐isobutylthioribose plus adenine. The latter reaction is not due to bacterial contamination, since the same results were obtained under sterile conditions and in chick embryo fibroblasts in culture. The inhibition of the virus‐induced cell transformation reported by us previously was due to (iBuS)5′ Ado rather than to the main metabolic product of this molecule in chick embryo fibroblasts.