Identification of Two Palmitoyl Groups in Octopus Rhodopsin
- 2 January 1997
- journal article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 65 (1) , 187-191
- https://doi.org/10.1111/j.1751-1097.1997.tb01897.x
Abstract
We determined the structure and site of fatty acid incorporated in octopus rhodopsin using a combination of fluorescence label and enzymatic cleavage methods in conjunction with fast-atom bombardment (FAB) mass spectrometry. A single peptide containing two adjacent cysteines, Cys337 and Cys338, was successfully isolated using the fluorescence from a dye conjugated to Cys345. The FAB mass spectrometric analysis of the peptide (323Phe-340Phe) revealed that two palmitoyl groups are linked to Cys337 and Cys338 via thioester bonds in octopus rhodopsin as in bovine rhodopsin.Keywords
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