EFFECTS OF TWO CHELATING AGENTS, OXINE AND DIETHYLDITHIOCARBAMATE (ANTABUSE), ON STAINABILITY AND MOTILITY OF HUMAN SPERMS
Open Access
- 1 October 1974
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 22 (10) , 981-985
- https://doi.org/10.1177/22.10.981
Abstract
Two different staining methods (the sulfide silver method of Timm and a fluorescence method using 2-methyl-8-hydroxyquinoline) revealed a similar distribution of heavy metals in human spermatozoa. Both methods were modified for the staining of spermatozoa. Diethyldithiocarbamate, a compound thought to be the active metabolite of disulfiram (an antialcoholic drug, Antabuse) prevented subsequent staining by these two methods. Low concentrations (1:10,000) of diethyldithiocarbamate in vitro immobilized spermatozoa within 2-4 hr but subsequent intravital staining with eosin indicated that more than 90% of the immobilized spermatozoa were still alive.Keywords
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