Cloning of Klebsiella pneumoniae pqq genes and PQQ biosynthesis in Escherichia coli

15 September 1990

journal article
Published by Oxford University Press (OUP) in FEMS Microbiology Letters

Vol. 71 (3) , 337-343
https://doi.org/10.1016/0378-1097(90)90244-k

Abstract

We have cloned genes from Klebsiella pneumoniae which are required for pyrroloquinoline quinone (PQQ) biosynthesis. The cloned 6.7 kb fragment can complement several chromosomal pqq mutants. Escherichia coli strains are unable to synthesize PQQ but E. coli strains containing the cloned 6.7 kb K. pneumoniae fragment can synthesize PQQ in large amounts and E. coli pts mutants can be complemented on minimal glucose medium by this clone.

Keywords

PYRROLOQUINOLINE QUINONE
KLEBSIELLA PNEUMONIAE
CLONING
PQQ GENE
GLUCOSE DEHYDROGENASE