Retrograde Support of Neuronal Survival Without Retrograde Transport of Nerve Growth Factor
- 22 February 2002
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 295 (5559) , 1536-1539
- https://doi.org/10.1126/science.1064913
Abstract
Application of nerve growth factor (NGF) covalently cross-linked to beads increased the phosphorylation of TrkA and Akt, but not of mitogen-activated protein kinase, in cultured rat sympathetic neurons. NGF beads or iodine-125–labeled NGF beads supplied to distal axons resulted in the survival of over 80% of the neurons for 30 hours, with little or no retrograde transport of iodine-125–labeled NGF; whereas application of free iodine-125–labeled NGF (0.5 nanograms per milliliter) produced 20-fold more retrograde transport, but only 29% of the neurons survived. Thus, in contrast to widely accepted theory, a neuronal survival signal can reach the cell bodies unaccompanied by the NGF that initiated it.Keywords
This publication has 28 references indexed in Scilit:
- Receptor binding, internalization, and retrograde transport of neurotrophic factorsCellular and Molecular Life Sciences, 2001
- Quantitative Imaging of Lateral ErbB1 Receptor Signal Propagation in the Plasma MembraneScience, 2000
- Spatially and Functionally Distinct Roles of the PI3-K Effector Pathway during NGF Signaling in Sympathetic NeuronsNeuron, 2000
- Rapid Retrograde Tyrosine Phosphorylation of trkA and Other Proteins in Rat Sympathetic Neurons in Compartmented CulturesThe Journal of cell biology, 1997
- Leukemia Inhibitory Factor and Nerve Growth Factor are Retrogradely Transported and Processed by Cultured Rat Sympathetic NeuronsDevelopmental Biology, 1994
- Temporal analysis of events associated with programmed cell death (apoptosis) of sympathetic neurons deprived of nerve growth factor.The Journal of cell biology, 1993
- Retrograde axonal transport of the GTP-binding protein Giα: A potential neurotrophic intra-axonal messengerNeuroscience Letters, 1991
- Quantitative demonstration of the retrograde axonal transport of endogenous nerve growth factorNeuroscience Letters, 1983
- Comparison between the retrograde axonal transport of nerve growth factor and tetanus toxin in motor, sensory and adrenergic neuronsBrain Research, 1975
- The retrograde axonal transport of nerve growth factorBrain Research, 1974