Tolerance of Trifolium subterraneum cultivars to low pH

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Abstract
Eleven cultivars of Trifolium subterraneum and Trifolium semipilosum cv. Safari were grown with adequate combined nitrogen for 27 days in flowing solution culture with controlled nutrient concentrations at constant pH values ranging from 3.5 to 6.5. A solution pH of 3.5 was lethal to all cultivars, but growth was in all cases vigorous at pH 4.0 (RGR 15.2-16.9 g 100 g-1 day-1). There were no significant effects of pH over the range of 4.0-6.5 on the yield of any clover cultivar. The results are discussed in relation to an earlier study suggesting greater tolerance of subterranean clover to pH values below 4.0. Phosphorus toxicity symptoms developed in all subterranean clover cultivars with the intensity of symptom development increasing with solution pH from 4.5 to 6.5. The concentration of phosphorus in the older leaves decreased as the solution pH was increased from 4.0 to 4.5, and then increased with further increase in pH, reaching values = 1.0%. Rates of phosphorus absorption followed a similar pattern of response to solution pH. Results are discussed with reference to previously reported effects of pH on phosphorus uptake.

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