STRAND SEPARATION AND SPECIFIC RECOMBINATION IN DEOXYRIBONUCLEIC ACIDS: BIOLOGICAL STUDIES
- 1 April 1960
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 46 (4) , 453-461
- https://doi.org/10.1073/pnas.46.4.453
Abstract
DNA from D. pneumoniae when heated to temperatures where all the molecules are denatured still retains transforming activity. The activity is increased by slowly cooling the denatured DNA as well as by increasing the ionic strength and concentration of DNA during the cooling period. The reunion of the strands is specific and has been shown to occur between populations of denatured molecules of mutant strains of D. pneumoniae. The reunion does not occur between the DNA strands of pneumococcus and that from Streptococcus D or from organisms with widely varying base ratios. Hybrid molecules in which one strand contains the mutant marker and the other strand derives from wild-type DNA are active in carrying out transformations with respect to the mutant property.Keywords
This publication has 16 references indexed in Scilit:
- Genetic transformation II. The significance of damage to the DNA moleculeBiochimica et Biophysica Acta, 1959
- Heterogeneity in Deoxyribonucleic Acids: I. Dependence on Composition of the Configurational Stability of Deoxyribonucleic AcidsNature, 1959
- The replication of DNA in Escherichia coliProceedings of the National Academy of Sciences, 1958
- THE DEPENDENCE OF PNEUMOCOCCAL TRANSFORMATION ON THE MOLECULAR WEIGHT OF DEOXYRIBOSE NUCLEIC ACIDProceedings of the National Academy of Sciences, 1958
- Interspecific reactions in bacterial transformation.1958
- Analysis of the Complex Sulfonamide Resistance Locus of PneumococcusCold Spring Harbor Symposia on Quantitative Biology, 1958
- Deoxyribonucleic acid incorporation by transformed bacteriaBiochimica et Biophysica Acta, 1957
- TRANSFORMATION REACTIONS BETWEEN PNEUMOCOCCUS AND THREE STRAINS OF STREPTOCOCCIThe Journal of Experimental Medicine, 1957
- Initiation of Bacterial TransformationNature, 1957
- STUDIES ON THE CHEMISTRY OF THE TRANSFORMING ACTIVITYThe Journal of Experimental Medicine, 1953