A Functional Interplay Between Aurora-A, Plk1 and TPX2 at Spindle Poles: Plk1 Controls Centrosomal Localization of Aurora-A and TPX2 Spindle Association

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Abstract
Aurora-A and Plk1 are centrosomal kinases involved in centrosome maturation and spindle assembly. The microtubule-binding protein TPX2 interacts with, and activates, Aurora-A. Here we have used RNA interference-mediated inactivation to investigate whether Aurora-A, Plk1 and TPX2 act independently or are part of one signalling cascade in spindle formation in mammalian cells. We have identified both specific, and overlapping, roles of each single regulator in centrosome maturation and spindle formation: (i) Aurora-A and TPX2 are required for centriole cohesion and spindle bipolarity; (ii) TPX2, besides its known role in microtubule organization, is also involved in centrosome maturation; (iii) finally, Plk1 controls the localization of Aurora-A to centrosomes, as well as TPX2 recruitment to microtubules. Based on these results therefore a hierachical functional relation between Plk1 and the Aurora-A/TPX2 pathway emerges.

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